SS-31 reconstitution calculator

SS-31 at a glance

10mg vial + 1mL BAC water = 25 units = 2.5mg — Frequency: daily, 12 weeks on, 4 weeks off — Best taken: any consistent time

Full SS-31 dosing reference — reconstitution per vial size, dose-to-units table, side effects and sources.

What it does

• Also known as Elamipretide — targets mitochondria and stabilizes cardiolipin, a key membrane lipid.
• Stabilizing that membrane lets existing mitochondria make more energy (ATP) per unit of oxygen and leak fewer free radicals, which lowers ongoing damage — it supports the mitochondria you already have rather than rebuilding damaged cells.
• FDA-approved (as Forzinity) specifically for Barth syndrome, a rare genetic mitochondrial disease.
• Studied more broadly for mitochondrial dysfunction linked to aging, heart failure, and eye disease.
• Uses beyond Barth syndrome remain investigational and are not FDA-approved.

Notes

FDA-approved only for Barth syndrome under the brand name Forzinity; other uses are investigational. Published clinical trials (Barth syndrome, mitochondrial myopathy) used continuous daily dosing, in some cases for 3+ years without a break — the FDA-approved protocol is not cycled. Separately, community wellness protocols commonly describe a cycled pattern instead: 12 weeks on, then 4 weeks off. Both patterns exist in real use; the daily frequency listed here matches the clinical evidence. The 10mg vial mixes to 10mg/mL. The 30mg and 50mg options are capped at ~3mL BAC water instead of diluted further, so they come out more concentrated (fewer units per dose) than the 10mg vial.

Side Effects

• Injection site redness, hardening, itching, pain, or bruising — most common in trials.
• Dose changes for reactions were uncommon (under 5%).
• No serious events clearly attributed to the drug.
• No cardiovascular signal in trials up to 24 weeks.

SS-31 vial sizes and concentrations

Standard water for each SS-31 vial size in the Dosify Me data, and the concentration it gives. One unit is 0.01 mL on a U-100 insulin syringe.

^* This size is conventionally mixed more concentrated rather than diluted further — fewer units per dose than smaller vials of this peptide.

Syringe units for the listed quantities, by vial size

Worked example for SS-31

1. The example vial: 50 mg of SS-31, mixed with 3.00 mL of bacteriostatic water.
2. Concentration: 50 mg ÷ 3.00 mL = 16.67 mg/mL.
3. One syringe unit is 0.01 mL, so it holds 16.67 mg/mL × 0.01 mL = 0.167 mg (166.67 mcg).
4. Example quantity: 2500 mcg = 2.5 mg.
5. Volume: 2.5 mg ÷ 16.67 mg/mL = 0.15 mL.
6. Syringe reading: 0.15 mL × 100 = 15 units.

Label numbers from the Dosify Me data, used to show the arithmetic. Not a recommendation of any quantity.

Frequently asked

How many units is 1 mg of SS-31?

At 10 mg/mL (the standard mix for vials up to 30 mg), 1 mg of SS-31 is 10.0 units on a 100-unit insulin syringe (0.100 mL).

How many units is 2.5 mg of SS-31?

At 10 mg/mL (the standard mix for vials up to 30 mg), 2.5 mg of SS-31 is 25.0 units on a 100-unit insulin syringe (0.250 mL).

How many units is 5 mg of SS-31?

At 10 mg/mL (the standard mix for vials up to 30 mg), 5 mg of SS-31 is 50.0 units on a 100-unit insulin syringe (0.500 mL).

How much bacteriostatic water do I add to a 10 mg vial of SS-31?

1.00 mL, which gives 10 mg/mL. Larger vials hold at most 3 mL of water, so they are mixed more concentrated and need fewer units per dose.

How much bacteriostatic water do I add to a 30 mg vial of SS-31?

3.00 mL, which gives 10 mg/mL. Larger vials hold at most 3 mL of water, so they are mixed more concentrated and need fewer units per dose.

How much bacteriostatic water do I add to a 50 mg vial of SS-31?

3.00 mL, which gives 16.67 mg/mL. This size holds at most 3 mL of water, so it is mixed more concentrated and the same dose is fewer units than from a smaller vial.

How often is SS-31 taken?

Daily, 12 weeks on, 4 weeks off. Any consistent time Typical cycle: 12 weeks on, 4 weeks off.

Related pages and guides

• SS-31 dosing reference
• How many units is 250 mcg?
• How much bacteriostatic water for a 5 mg vial?
• U-100 vs U-50 insulin syringe for peptides
• Peptide injection site rotation
• All peptide guides

Other calculators

General peptide calculator · BAC water calculator · Peptide dosing chart · How to reconstitute peptides · mcg to units chart