NAD+ reconstitution calculator

NAD+ at a glance

500mg vial + 3mL BAC water = 30 units = 50mg — Frequency: daily or 2-3x weekly — Best taken: slow injection is reported to reduce stinging

Full NAD+ dosing reference — reconstitution per vial size, dose-to-units table, side effects and sources.

What it does

• Not a peptide — it's a coenzyme found in every cell, essential for energy metabolism and DNA repair. Included here because it's commonly stacked alongside peptide protocols.
• Popular in injection/IV-therapy clinics for energy, recovery, and anti-aging claims, though rigorous human clinical trial evidence for injectable use is limited.
• Typically mixed with just 3mL of BAC water regardless of vial size — a 1000mg vial isn't diluted further, so it comes out roughly twice as concentrated as a 500mg vial (about 333mg/mL vs 167mg/mL).
• NAD+ injections are known for a burning/stinging sensation — some people dilute further than the standard 3mL (up to 6mL) specifically to reduce this, at the cost of a larger injection volume.
• The 50-100mg amounts here are subcutaneous community figures; IV clinic sessions use 250-1000mg and are not interchangeable.

Notes

Both the 500mg and 1000mg vials are conventionally mixed with only 3mL BAC water — same water volume as most other peptides here, but far more total mg, so the resulting solution is much more concentrated. Doses are large in absolute mg (50-100mg) compared to typical mcg-dosed peptides, so the injection volume is bigger than usual, and mild stinging/flushing is commonly reported when injected quickly.

Side Effects

• Injection site stinging and soreness — very common; slower injection and more dilution are reported to help.
• Niacin-like flush within 10-20 minutes, gone within an hour.
• Nausea, headache, fatigue for 1-3 hours.
• Rapid IV infusion only: low blood pressure, fast heart rate, chest tightness.

NAD+ vial sizes and concentrations

Standard water for each NAD+ vial size in the Dosify Me data, and the concentration it gives. One unit is 0.01 mL on a U-100 insulin syringe.

^* This size is conventionally mixed more concentrated rather than diluted further — fewer units per dose than smaller vials of this peptide.

Syringe units for the listed quantities, by vial size

Worked example for NAD+

1. The example vial: 1000 mg of NAD+, mixed with 3.00 mL of bacteriostatic water.
2. Concentration: 1000 mg ÷ 3.00 mL = 333.33 mg/mL.
3. One syringe unit is 0.01 mL, so it holds 333.33 mg/mL × 0.01 mL = 3.333 mg (3333.33 mcg).
4. Example quantity: 50000 mcg = 50 mg.
5. Volume: 50 mg ÷ 333.33 mg/mL = 0.15 mL.
6. Syringe reading: 0.15 mL × 100 = 15 units.

Label numbers from the Dosify Me data, used to show the arithmetic. Not a recommendation of any quantity.

Frequently asked

How many units is 50 mg of NAD+?

At 166.67 mg/mL (the standard mix for vials up to 500 mg), 50 mg of NAD+ is 30.0 units on a 100-unit insulin syringe (0.300 mL).

How many units is 75 mg of NAD+?

At 166.67 mg/mL (the standard mix for vials up to 500 mg), 75 mg of NAD+ is 45.0 units on a 100-unit insulin syringe (0.450 mL).

How many units is 100 mg of NAD+?

At 166.67 mg/mL (the standard mix for vials up to 500 mg), 100 mg of NAD+ is 60.0 units on a 100-unit insulin syringe (0.600 mL).

How much bacteriostatic water do I add to a 500 mg vial of NAD+?

3.00 mL, which gives 166.67 mg/mL. Larger vials hold at most 3 mL of water, so they are mixed more concentrated and need fewer units per dose.

How much bacteriostatic water do I add to a 1000 mg vial of NAD+?

3.00 mL, which gives 333.33 mg/mL. This size holds at most 3 mL of water, so it is mixed more concentrated and the same dose is fewer units than from a smaller vial.

How often is NAD+ taken?

Daily or 2-3x weekly. Any time, slow injection is reported to reduce stinging Typical cycle: ~2 weeks on, then break; repeat 2–3×/year.

Related pages and guides

• NAD+ dosing reference
• How many units is 250 mcg?
• How much bacteriostatic water for a 5 mg vial?
• U-100 vs U-50 insulin syringe for peptides
• Peptide injection site rotation
• All peptide guides

Other calculators

General peptide calculator · BAC water calculator · Peptide dosing chart · How to reconstitute peptides · mcg to units chart