FOXO4-DRI

Reconstitution

Every vial size below is mixed to the same concentration, 3.33 mg/mL, so the syringe reads the same number of units for a given dose no matter which vial you bought.

Dose to syringe units

On a standard 100-unit (1 mL) insulin syringe, at the concentration above:

Amounts listed in the Dosify Me data: 250–500 mcg. Frequency: daily, 16-week ladder. Timing: evening.

All vial sizes reconstitute to 3.33mg/mL (33.3mcg/unit) so the syringe reads the same regardless of size.

Reading units for FOXO4-DRI

• Syringe units measure volume, not the amount of FOXO4-DRI: on a U-100 insulin syringe 100 units is 1 mL.
• The vial is labelled in mg while quantities are often written in mcg: 1 mg is 1000 mcg, so a 10 mg vial holds 10000 mcg.

Check one row of the table above: 250 mcg is 0.25 mg; 0.25 mg ÷ 3.33 mg/mL = 0.075 mL; 0.075 mL × 100 = 7.5 units.

What it does

• A senolytic peptide designed to selectively trigger death of aged ("senescent") cells, studied for anti-aging effects.
• Only studied in mice so far — the well-known 2017 study reversed some aging signs in older mice.
• No human trials or human dosing data exist — this is very early-stage research that warrants significant caution.
• One mouse study found this peptide reduced chemotherapy-related toxicity — a side-effect-reduction finding, not evidence of cancer prevention or treatment.
• Some community protocols instead use short pulses of much larger doses; no human data supports either.

Why it is listed under Longevity & Mitochondrial, Cancer Risk & Prevention

• Longevity & Mitochondrial: Senolytic targeting aged cells for anti-aging effects in mice.
• Cancer Risk & Prevention: One mouse study shows it may reduce chemotherapy-related toxicity — a side-effect finding, not evidence it prevents or treats cancer.

What users report

Anecdotal and early-research reports, kept apart from the evidence above. Not clinically established.

• Users report feeling more energetic and 'refreshed' after a course, the anecdotal counterpart to the mouse rejuvenation study.
• Some report better hair and skin quality and improved exercise recovery.
• Claims of reversing aging or clearing senescent cells in people are unverified — there is no human data at all.

Side effects

• Injection site burning, fatigue, muscle soreness, nausea — anecdotal.
• Mild flu-like feeling, possibly from senescent-cell clearance; unconfirmed.
• No side effects in the 2017 mouse study — mouse data only.
• Theoretical long-term DNA-repair concern; unstudied beyond 30 days even in animals.

FOXO4-DRI calculator

Different vial or dose? Open the FOXO4-DRI reconstitution calculator, pre-filled with these numbers.

Frequently asked

How many units is 250 mcg of FOXO4-DRI?

At 3.33 mg/mL (the concentration every vial size mixes to), 250 mcg of FOXO4-DRI is 7.5 units on a 100-unit insulin syringe (0.075 mL).

How many units is 375 mcg of FOXO4-DRI?

At 3.33 mg/mL (the concentration every vial size mixes to), 375 mcg of FOXO4-DRI is 11.2 units on a 100-unit insulin syringe (0.113 mL).

How many units is 500 mcg of FOXO4-DRI?

At 3.33 mg/mL (the concentration every vial size mixes to), 500 mcg of FOXO4-DRI is 15.0 units on a 100-unit insulin syringe (0.150 mL).

How much bacteriostatic water do I add to a 10 mg vial of FOXO4-DRI?

3.00 mL, which gives 3.33 mg/mL. Other vial sizes use proportionally more or less water so the syringe reads the same.

How often is FOXO4-DRI taken?

Daily, 16-week ladder. Evening Typical cycle: 16-week daily ladder (community protocol; no human data).

Sources

• FOXO4-DRI Alleviates Age-Related Testosterone Secretion Insufficiency in Aged Mice (PMC)
• Targeted apoptosis of senescent cells by FOXO4-DRI (Baar et al., Cell 2017)
• A FOXO4 inhibitory peptide limits chemotoxicity in mice (Cancer Discovery, AACR)
• Senolytic FOXO4-DRI removes senescent cells from human chondrocytes (Frontiers in Bioeng. & Biotech.)
• The p53 transactivation domain is the target of FOXO4 and FOXO4-DRI (Nature Communications)

Related pages and guides

• FOXO4-DRI calculator
• How many units is 250 mcg?
• How much bacteriostatic water for a 5 mg vial?
• U-100 vs U-50 insulin syringe for peptides
• Peptide storage and refrigeration
• All peptide guides